Improved SSA-Based GRU Neural Network for BDS-3 Satellite Clock Bias Forecasting.

Satellite clock error is a key factor affecting the positioning accuracy of a global navigation satellite system (GNSS). In this paper, we use a gated recurrent unit (GRU) neural network to construct a satellite clock bias forecasting model for the BDS-3 navigation system. In order to further improve the prediction accuracy and stability of the GRU, this paper proposes a satellite clock bias forecasting model, termed ITSSA-GRU, which combines the improved sparrow search algorithm (SSA) and the GRU, avoiding the problems of GRU's sensitivity to hyperparameters and its tendency to fall into local optimal solutions. The model improves the initialization population phase of the SSA by introducing iterative chaotic mapping and adopts an iterative update strategy based on t-step optimization to enhance the optimization ability of the SSA. Five models, namely, ITSSA-GRU, SSA-GRU, GRU, LSTM, and GM(1,1), are used to forecast the satellite clock bias data in three different types of orbits of the BDS-3 system: MEO, IGSO, and GEO. The experimental results show that, as compared with the other four models, the ITSSA-GRU model has a stronger generalization ability and forecasting effect in the clock bias forecasting of all three types of satellites. Therefore, the ITSSA-GRU model can provide a new means of improving the accuracy of navigation satellite clock bias forecasting to meet the needs of high-precision positioning.

Siamese Transformer-Based Building Change Detection in Remote Sensing Images.

To address the challenges of handling imprecise building boundary information and reducing false-positive outcomes during the process of detecting building changes in remote sensing images, this paper proposes a Siamese transformer architecture based on a difference module. This method introduces a layered transformer to provide global context modeling capability and multiscale features to better process building boundary information, and a difference module is used to better obtain the difference features of a building before and after a change. The difference features before and after the change are then fused, and the fused difference features are used to generate a change map, which reduces the false-positive problem to a certain extent. Experiments were conducted on two publicly available building change detection datasets, LEVIR-CD and WHU-CD. The F1 scores for LEVIR-CD and WHU-CD reached 89.58% and 84.51%, respectively. The experimental results demonstrate that when utilized for building change detection in remote sensing images, the proposed method exhibits improved robustness and detection performance. Additionally, this method serves as a valuable technical reference for the identification of building damage in remote sensing images.

Injectable self-crosslinking hydrogels based on hyaluronic acid as vitreous substitutes.

After vitrectomy, the ideal vitreous substitute should be implanted to maintain the normal function of the eye. However, the existing materials (such as silicone oil, air, perfluorocarbons, etc.) still have some shortcomings and cannot fully meet the clinical needs. In this study, thiolated hyaluronic acid (SH-HA) was prepared based on hyaluronic acid. The SH-HA hydrogel was formed by a simple transformation of the sulfhydryl group to the disulfide bond, which had high transparency, controllable swelling property, suitable mechanical strength, excellent biocompatibility and similar physical and chemical properties to natural vitreous. SH-HA hydrogel was filled into the eyes of experimental rabbits to replace their own vitreous after vitrectomy. During the 90 days follow-up period, SH-HA hydrogel showed excellent intraocular compatibility, maintained normal intraocular pressure (IOP), and no cataract, endophthalmitis, retinal detachment and other complications were observed. In general, SH-HA hydrogel has great potential as a vitreous substitute.

A self-healing and injectable hydrogel based on water-soluble chitosan and hyaluronic acid for vitreous substitute.

Vitreous, an essential dioptric medium for the human eyes, must be filled with artificial materials once damaged. Carboxymethyl chitosan (CMCTS) is one of the most important water-soluble chitosan derivatives with improved biocompatibility and biodegradability. In this study, oxidized hyaluronic acid (OHA) was prepared as crosslinking reagent. CMCTS and OHA were used to develop a biocompatible, self-repairing and in-situ injectable hydrogel for vitreous substitutes. Results showed the hydrogel with controllable swelling properties, high transparency, acceptable cytocompatibility on mouse fibroblast L929 and histocompatibility in vivo. Furthermore, hydrogel was injected in-situ into the vitreous cavity after vitrectomy on New Zealand Rabbits, no significant and persistent adverse effects were observed during the 90-day follow-up period. In addition, the hydrogel maintained intraocular pressure of the operated eyes and the inherent position of the retina. Collectively, this injectable, biodegradable, nontoxic hydrogel possessed enormous potential to become a vitreous substitute material.

Experimental Study of a Signal Modulation Method to Improve eLORAN Data Channel Communications.

There are mainly two types of data modulation methods used for enhanced LOng-RAnge Navigation (eLORAN) systems: pulse position modulation (PPM) and supernumerary interpulse modulation (SIM). The typical application for PPM is tri-state PPM (3S-PPM), also known as Eurofix. The typical application for SIM is ninth pulse modulation. Both of these methods are phase modulation methods. Phase modulation coding, a very mature technology, is used at present. To achieve a better demodulation success rate of eLORAN digital modulation signals at longer distances, a method of using the transmitting station duplex mode to transmit a digital modulation pulse group after LORAN-C transmitting a pulse group is proposed to realize modulation pulse on-off modulation. In this method, a broadcasting experiment was performed on the BPL (The call sign of eLORAN time service system in China) broadcaster station. After monitoring, a good receiving demodulation effect was initially obtained.

The feasibility study of an in situ marine polysaccharide-based hydrogel as the vitreous substitute.

Although various biopolymers and synthetic compounds have been proposed and tested as the vitreous substitutes, no ideal material has been identified yet. In the present study, we developed an in situ-formed hydrogel by crosslinking hydroxypropyl chitosan with alginate dialdehyde. Physical properties of the hydrogel were studied and the cytotoxicity was evaluated using L929 fibroblasts, rabbit corneal endothelial cells and retinal pigment epithelial cells. In a preliminary in vivo study, the hydrogel was employed as vitreous substitute after vitrectomy surgery on rabbits and multiple parameters indicating biosafety and biocompatibility were measured and analyzed postoperatively. Our results showed that the refractive index, transmittance, pH value and density of the hydrogel were similar to those of human vitreous. Cytotoxicity tests demonstrated the hydrogel to be nontoxic to all of the three cell lines selected. Using the rabbit model, we showed that the hydrogel could form in situ and postoperative analysis of slit-lamp observation, intraocular pressure, corneal endothelium examination, B-scan ultrasound and fundus photography showed no significant adverse reactions in the operated eyes during the 90-day follow-up. However, electroretinogram and histopathological examinations indicated minor vision decline and decrease of the densities of cones and rods in the operated rabbit eyes. Collectively, our study suggested that the in situ-formed hydrogel could potentially be used as a vitreous substitute, with its long-term safety and efficacy to be further assessed. © 2018 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 106A: 1997-2006, 2018.

Evaluation of a photocrosslinkable hydroxyethyl chitosan hydrogel as a potential drug release system for glaucoma surgery.

Hydroxyethyl chitosan (HECTS) is a critical derivative of chitosan that has been widely used as biomedical materials due to great water-solubility and excellent biocompatibility. Here, photosensitive hydroxyethyl chitosan was synthesized by introducing azide group on NH2 of HECTS (HECTS-AZ), afterwards FTIR and 1H NMR spectra were detected to confirm the formation of HECTS-AZ. The solution of HECTS-AZ can achieve a sol-gel transition through UV irradiation for 30 s. The evaluation of biocompability and biodegradability in vivo was conducted in rats, visual and pathological examinations exhibited the HECTS-AZ has excellent biocompability and degradation time of the hydrogel is more than 14 weeks. Furthermore, HECTS-AZ hydrogel as an ocular drug delivery system loading heparin was prepared to implant under sclera of rabbit after glaucoma filtration surgery (GFS). The experimental results demonstrated the heparin loaded hydrogel can effectively maintain filtration bleb and lowing intraocular pressure (IOP) after GFS for prolonged time. Besides, obvious inflammatory reactions and side effects have not been observed in ocular during the experimental period. In conclusion, the HECTS-AZ hydrogel is a potential drug delivery device for the treatment of glaucoma and other ocular diseases.

Therapeutic efficiency of tissue-engineered human corneal endothelium transplants on rabbit primary corneal endotheliopathy.

To evaluate the therapeutic efficiency of tissue-engineered human corneal endothelia (TE-HCEs) on rabbit primary corneal endotheliopathy (PCEP), TE-HCEs reconstructed with monoclonal human corneal endothelial cells (mcHCECs) and modified denuded amniotic membranes (mdAMs) were transplanted into PCEP models of New Zealand white rabbits using penetrating keratoplasty. The TE-HCEs were examined using diverse techniques including slit-lamp biomicroscopy observation and pachymeter and tonometer measurements in vivo, and fluorescent microscopy, alizarin red staining, paraffin sectioning, scanning and transmission electron microscopy observations in vitro. The corneas of transplanted eyes maintained transparency for as long as 200 d without obvious edema or immune rejection. The corneal thickness of transplanted eyes decreased gradually after transplanting, reaching almost the thickness of normal eyes after 156 d, while the TE-HCE non-transplanted eyes were turbid and showed obvious corneal edema. The polygonal corneal endothelial cells in the transplanted area originated from the TE-HCE transplant. An intact monolayer corneal endothelium had been reconstructed with the morphology, cell density and structure similar to those of normal rabbit corneal endothelium. In conclusion, the transplanted TE-HCE can reconstruct the integrality of corneal endothelium and restore corneal transparency and thickness in PCEP rabbits. The TE-HCE functions normally as an endothelial barrier and pump and promises to be an equivalent of HCE for clinical therapy of human PCEP.

Fabrication and characters of a corneal endothelial cells scaffold based on chitosan.

A novel chitosan-based membrane that made of hydroxyethyl chitosan, gelatin and chondroitin sulfate was used as a carrier of corneal endothelial cells. The characteristics of the blend membrane including transparency, equilibrium water content, ion and glucose permeability were determined. The results showed that the optical transparency of the membrane was as good as the natural human cornea. The water content of this scaffold was 81.32% which was remarkably close to the native cornea. The membrane had a good ion permeability and its glucose permeability was even higher than natural human cornea. The cultured rabbit corneal endothelial cells formed a monolayer on the membrane. The results demonstrated that the membrane was suitable for corneal endothelial cells to attach and grow on it. In addition, the membranes in vivo could be degraded steadily with less inflammation and showed a good histocompatibility. These results demonstrated that the hydroxyethyl chitosan-chondroitin sulfate-gelatin blend membrane can potentially be used as a carrier for corneal endothelial cell transplantation.

An in situ formed biodegradable hydrogel for reconstruction of the corneal endothelium.

Biodegradable hydrogels are important biomaterials for tissue engineering and drug delivery. For the purpose of corneal regenerative medicine, we describe an in situ formed hydrogel based on a water-soluble derivative of chitosan, hydroxypropyl chitosan (HPCTS), and sodium alginate dialdehyde (SAD). Periodate oxidized alginate rapidly cross-links HPCTS due to Schiff's base formation between the available aldehyde and amino groups. Hydrogel cytotoxicity, degradability and histocompatibility in vivo were examined. The potential of the composite hydrogel for corneal endothelium reconstruction was demonstrated by encapsulating corneal endothelial cells (CECs) to grow on Descemet's membranes. The results demonstrate that the composite hydrogel was both non-toxic and biodegradable and that CECs transplanted by the composite hydrogel could survive and retain normal morphology. These results provide an opportunity for corneal endothelium reconstruction based on tissue engineering by the in situ formed composite hydrogel.

Preparation and properties of a chitosan-based carrier of corneal endothelial cells.

A novel chitosan-based membrane that was made of hydroxypropyl chitosan, gelatin and chondroitin sulfate was used as a carrier of corneal endothelial cells. The characteristics of the blend membrane, such as transparency, equilibrium water content, permeability, mechanical properties, protein absorption ability, hydrophilicity and surface morphology, were determined. To study the effects of the membrane on cell attachment and growth, rabbit corneal endothelial cells were cultured on this artificial membrane. The biodegradability and biocompatibility of the blend membrane were in vivo evaluated by its implantation into the muscle of the rats. Glucose permeation results demonstrated that the blend membrane had higher glucose permeability than natural human cornea. Scanning electron microscopy (SEM) analysis of the membranes demonstrated that no fibrils were observed. As a result, the optical transparency of the membrane was as good as the natural human cornea. The average value of tensile strength of the membrane was 13.71 MPa for dry membrane and 1.48 MPa for wet membrane. The value of elongation at break of the wet was 45.64%. The cultured rabbit corneal endothelial cells formed a monolayer on the blend membrane which demonstrated that the membrane was suitable for corneal endothelial cells to attach and grow. In addition, the membranes in vivo showed a good bioabsorption property. The mild symptoms of inflammation at sites of treatment could be resolved as the implant was absorbed by the host. The results of this study demonstrated that the hydroxypropyl chitosan-chondroitin sulfate-gelatin blend membrane can potentially be used as a carrier for corneal endothelial cell transplantation.